WebJun 1, 2024 · For phosphorescence imaging or phosphorescence lifetime measurements in HK-2 cells, all cells were cultured on glass-base dishes. After 2-hour incubation with BTPDM1, the media was changed to those containing antimycin A (10 μM), followed by PLIM. PLIM and fluorescence lifetime imaging microscopy configurations WebJan 24, 2024 · Application of lifetime-based techniques, referred to as fluorescence (FLIM) and phosphorescence (PLIM) lifetime imaging, makes possible to use the lifetime as a function of some particular biologically relevant microenvironment parameters. Thus, FLIM and PLIM should be regarded as “functional imaging ” techniques, as opposite to various ...
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WebAug 1, 2024 · Generally, we categorize them into the following sections, including (i) compartmentalization staining of cells, (II) phosphorescence lifetime imaging microscopy, (III) two photon bioimaging and (IV) bioimaging of hypoxic tumors. 2. Compartmentalization staining of cells using phosphorescent Ir (III) complexes WebJul 21, 2024 · The FLIM method. Fluorescence lifetime imaging microscopy (FLIM) exploits the inherent properties of fluorescent dyes to produce an image. Fluorescent molecules have become essential tools for cell biologists. They are widely used in microscopy to visualize many different structures, targets and dynamic processes within cells, tissues, … philips 48oled806/12 zwart
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WebMay 1, 2024 · Two-photon Phosphorescence Lifetime Microscopy (2PLM) is an emerging nonlinear optical technique that has great potential to improve our understanding of the … WebJul 1, 2009 · Fluorescence lifetime imagingmicrosopy (FLIM) is a non-invasive technique in which an image of a fluorescent probe or marker can be obtained in a cell without compromising or damaging the cell. The combination of scanning laser beams and powerful computers has advanced the field rapidly over the past 20 years. WebTwo-photon Phosphorescence Lifetime Microscopy (2PLM) is an emerging nonlinear optical technique that has great potential to improve our understanding of the basic … philips 48 oled 806 recensioni