Fitc偶联annexin-v凋亡检测试剂盒

WebMethod. 1. Cell incubation with Annexin V-FITC. 1.1 Induce apoptosis via the desired method. 1.2 Collect 1–5 x 10 5 cells by centrifugation. 1.3 Resuspend cells in 500 µL of 1X Annexin V binding buffer. 1.4 Add 5 µL of annexin V-FITC and 5 µL of propidium iodide (PI, optional). 1.5 Incubate at room temperature for 5 min in the dark. http://www.bio-review.com/fitc-labeling-kit/

Annexin V/7-AAD 凋亡检测试剂盒 － 丁香通 - biomart.cn

WebFormat. Kit. Kit Contents. Contains: • Annexin V Binding Buffer (10X), 1 bottle (30 mL) • Annexin V-FITC conjugate, 1 vial (0.25 mL) • Propidium Iodide Staining Solution, 1 vial (0.5 mL) Note: Fixable Viability Dye eFluor 450 is not recommended for use with Annexin V Apoptosis Detection Kits. Product Type. WebTransfer 100 µL of cell suspension in 5 ml test tube. 3. Add 5 µL of FITC Annexin V. 4. Add 10 µL of PI solution (Cat. No. 421301) or 7-AAD (Cat. No. 420403/420404). 5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark. 6. Add 400 µL of Annexin V Binding Buffer (Cat. No. 422201) to each tube. canal towpath georgetown https://martinezcliment.com

Annexin V-FITC/7-AADApoptosis Detection Kit

Web在细胞凋亡过程中，PS转位到质膜的外叶，当钙存在时，可通过与荧光染料标记的Annexin V结合，通过流式细胞术和细胞成像进行检测。 BD Biosciences提供几种常见形式的Annexin V，如用于紫色激光的FITC、PE、BD Horizon™ BV421和用于紫外激光的BD Horizon™ BUV395。随着这些 ... WebMar 28, 2024 · 高品质 Annexin V/7-AAD 凋亡检测试剂盒. 北京义翘神州（Sino Biological Inc.）自主研发的高品质 Annexin V/7-AAD 凋亡检测试剂盒以 Annexin V 联合 7-AAD/PI 的双染法（提示：双染法不可区分晚期凋亡和细胞坏死，建议同时采用两种以上方法综合判断凋亡状态），操作简单 ... WebAnnexin V是一种分子量为35-36kD的Ca2 + 依赖性磷脂结合蛋白，能与细胞凋亡过程中翻转到膜外的磷脂酰丝氨酸（PS）高亲和力特异性结合。在正常细胞中，磷脂酰丝氨酸（PS）只分布在细胞膜脂质双层的内侧，而在细胞凋亡早期，细胞膜中的磷脂酰丝氨酸（PS）由脂膜 ... canal toys airbrush kitty chaton

Annexin V-FITC 凋亡检测试剂盒 Sigma-Aldrich

Category:Annexin V-FITC/PI细胞凋亡检测试剂盒-爱必信（absin）官网

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Fitc偶联annexin-v凋亡检测试剂盒

Annexin V/7-AAD 凋亡检测试剂盒 － 丁香通 - biomart.cn

Web英文名字 LinKine™ FITC Labeling Kit. 中文名称 LinKine™ FITC 偶联试剂盒. 产品货号 KTL0210. 规格 3*20ug. 背景资料： 异硫氰酸荧光素（FITC）是荧光素的衍生物，广泛应用于流式细胞术。 FITC是用异硫氰酸酯反应性基团（-N = C = S）官能化的原始荧光素分子，取代结构底部 ... WebAnnexin V是检测细胞早期凋亡的常用方法之一。实验操作虽然不算难，想要获得漂亮的流式结果图却并不容易，任何一个细节的疏忽都可能导致实验结果不理想。 本篇文章，带大家了解实验过程中的注意事项，及不能忽视的实验细节。 01 实验原理

Fitc偶联annexin-v凋亡检测试剂盒

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Web货号: BMS500FI-100. 使用用于进行流式细胞分析的 eBioscience 和 Annexin V 细胞凋亡检测试剂盒，可轻松区分早期和晚期细胞凋亡。. 这些试剂盒利用活力染料偶联膜联蛋白 V 标记细胞外膜上的磷脂酰丝氨酸（PS；细胞凋亡早期标记物）。. 在晚期细胞凋亡中，随着细胞 ... WebDead Cell Apoptosis Kit with Annexin V FITC and PI, for flow cytometry: Kit Contents: Contains 1 vial of annexin V, FITC conjugate (250 µL ), 1 vial of propidium iodide (PI, 100 µL), and 1 bottle of annexin binding buffer (5X …

Web实验笔记丨流式检测细胞凋亡之Annexin V 检测细胞凋亡. Annexin V 是检测细胞凋亡的灵敏指标之一。. 它是一种磷脂结合蛋白，可以与早期凋亡细胞的胞膜结合，而细胞质膜的改变是细胞发生凋亡时最早的改变之一。. 在细胞发生凋亡时，膜磷脂酰丝氨酸 (PS) 由质膜 ... Web3. 加入5-15µg纯化的重组Annexin V。注意，不同的细胞系，以及凋亡的不同阶段，其Annexin V位点 饱和所需要的纯化的重组Annexin V含量不同。某些情况下，为达到最好效果，可以减少细胞数量， 0.5´105个细胞加5-15µg纯化的重组Annexin V。 4. 轻轻混匀，室温反应15分钟。 5.

WebDocuments. 产品描述. 本试剂盒用于检测细胞凋亡早期的发生，其中Annexin V为胞内蛋白膜联蛋白家族成员，以钙离子依赖的方式选择性与磷脂酰丝氨酸 (PS)结合。. PS正常分布在细胞膜内侧，即与细胞浆相邻的一侧。. 在细胞发生凋亡的早期，不同类型的细胞都会把PS ... WebCatalog number: A13199. Achieve quick and reliable detection of early cell apoptosis with Annexin V stand-alone conjugates for apoptosis detection. Annexin V conjugates offer up to 100-fold difference in fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry. Contains 1 vial of annexin V, FITC conjugate.

WebAnnexin V-FITC Microscopy Staining Protocol. 1. Wash cells twice with 1X PBS. 2. Wash cells once with 1X Annexin V Binding Buffer. 3. Stain cells with Annexin V-FITC diluted 1:10 in 1X Annexin V Binding Buffer for 15 min at RT. We typically use 2 ml total volume per Labtek II chamber slide (i.e., 200 µl Annexin V-FITC and 1.8 ml 1X Binding Buffer.

WebFlow Cytometric Analysis of Annexin V-FITC staining. Jurkat cells were untreated (left panels), treated for 5 hours (middle panels) or 18 hours (right panels) with 4μMCamptothecin (Sigma,Cat.No C9911). Cells were incubated with Annexin V-FITC and 7-AAD and analyzed by flow cytometry. Untreated cells were primarily Annexin V-FITC … canal toys tie dye studioWeb悬浮细胞收集：离心5分钟；贴壁细胞：用不含EDTA的胰酶消化收集后（注：胰酶消化时间不宜过长，否则会影响细胞膜上磷脂酰丝氨酸与Annexin V-FITC的结合），于室温2000rpm离心5～10分钟，收集细胞；. （3）细胞洗涤：用预冷1×PBS（4℃）重悬细胞一次，2000rpm离心5 ... fisher price musical instruments gameWeb推荐的实验流程. FITC Annexin V is a sensitive probe for identifying apoptotic cells, binding to negatively charged phospholipid surfaces (Kd of ~5 x 10e-2) with a higher affinity for phosphatidylserine (PS) than most other phospholipids. FITC Annexin V binding is calcium dependent and defined calcium and salt concentrations are required ... canal tracts brinnonWebTransfer 100 µL of cell suspension in 5 ml test tube. 3. Add 5 µL of FITC Annexin V. 4. Add 10 µL of PI solution (Cat. No. 421301) or 7-AAD (Cat. No. 420403/420404). 5. Gently vortex the cells and incubate for 15 min at RT (25°C) in the dark. 6. Add 400 µL of Annexin V Binding Buffer (Cat. No. 422201) to each tube. canal tour in amsterdamWebAnnexin-V（膜联蛋白-V）是一种分子量为35-36KD的Ca2+依赖性磷脂结合蛋白，能与PS高亲和力结合。. 用荧光素（FITC、Alexa Fluor488等）标记的Annexin-V作为探针，利用流式细胞仪或荧光显微镜可检测细胞凋亡的发生；碘化丙啶 (propidine iodide, PI)是一种核酸染料，它不能透过 ... fisher price musical instruments toysWebAnnexin V is a Ca 2+-dependent phospholipid binding protein. This protein can bind to a variety of phospholipids, but it has the highest affinity for phosphatidylserine (PS). The ApoDETECT Annexin V-FITC Kit binds to … fisher price musical lion walker toys r usWebannexin v- fitc/pi 凋亡检测试剂盒.由晶抗生物整理，包括annexin v- fitc/pi 凋亡检测试剂盒.使用说明书、应用范围、使用注意事项及操作步骤等资讯。 欢迎莅临上海晶抗生物工程有限公司官方网站！ canal tours in venice